Long-term effects of the glucocorticoid receptor modulator cort 113176 in murine motoneuron degeneration.

Long-term effects of the glucocorticoid receptor modulator cort 113176 in murine motoneuron degeneration.

The Wobbler rat spinal cord motoneurons showed vacuolization especially, glial reaction, inflammation and abnormal glutamatergic parameters. Wobblers also showed a deficit of motor performance. This condition resembles amyotrophic lateral sclerosis (ALS). Wobbler mice also showed higher levels of corticosterone in the blood, adrenal and brain plus adrenal hypertrophy, suggesting that increased glucocorticoid prime chronic spinal cord nerve inflammation.

Therefore, we analyzed if treatment Wobbler mice with the glucocorticoid receptor (GR) antagonist reduced CORT113176 mentioned disorders. 30 mg / kg CORT113176 administered daily for 3 weeks reduced motoneuron vacuolation, decrease in astro and microgliosis, lowers inflammatory mediators group of high mobility box 1 protein (HMGB1), pulses like receptor 4, myeloid differentiation primary response 88 (MyD88), p50 subunit factor nuclear kappa B (NF), tumor necrosis factor (TNF) receptor, and interleukin 18 (IL18) compared with untreated Wobblers. CORT113176 improve survival signal pAkt (serine-threonine kinases) and a decrease in phosphorylated death signal Trash-N-terminal kinase (pJNK), symptoms of anti-apoptotic.

There was a moderate positive effect on glutamine synthase and astrocyte glutamate transporters, showed a decrease in glutamate excitotoxicity. In these pre-clinical studies, Wobblers receive CORT113176 show increased resistance to fatigue in the rota rod test and atrophy of the lower front leg in Week 2- 3. Therefore, the long-term treatment with attenuated CORT113176 degeneration and inflammation, improve motor performance and decrease foot deformities. Antagonism of GR may be a potential therapeutic value for neurodegenerative diseases.

Long-term effects of the glucocorticoid receptor modulator cort 113176 in murine motoneuron degeneration.
Long-term effects of the glucocorticoid receptor modulator cort 113176 in murine motoneuron degeneration.

resistin expression in human monocytes is controlled by two SNPs associated promoter binding mediation NFkB p50 / p50 and C-methylation.

Resistin is a key cytokine associated with metabolic and inflammatory diseases. Especially in East Asian populations, the level of expression is strongly influenced by genetic polymorphisms. The mechanism and functional implications of this genetic control remains unknown. By using the test reporter, EMSA, the study of inhibition, sequencing of bisulfite, CHIP-Seq and gene-editing we showed that the homodimer p50 / p50 is known to act as a repressor for a number of genes of pro-inflammation plays a central role in the genetic regulation of resistin in monocytes along with promoter methylation.

RETN common haplotype in the p50 / p50 offset constitutive expression by binding to the promoter. In a variant haplotypes Asia but this interaction is disrupted by the A allele of rs3219175. SNP is a very close relationship with rs34861192, a SNP CpG, located 280 bp upstream of the supply site C-allele-specific methylation.

rs34861192 lies in the region of 100 bp was found methylated haplotype similarities but not in Asia, so that the latter have a higher basal expression, which also associates with increased histone acetylation (H3K27ac). genotype associations in cohort data from 200 East Asians revealed a significant association between this haplotype and plasma levels of factors such as TGF-b, S100B, sRAGE and IL-8 as well as the number of myeloid DC.

NF kappaB p105/p50 Antibody

AF6221-200ul 200ul
EUR 350

Phospho- NF- kappaB p105/p50 (Ser927) Antibody

ABF3217 100 ug
EUR 525.6

Phospho- NF- kappaB p105/p50 (Ser932) Antibody

ABF3218 100 ug
EUR 525.6

Phospho- NF- kappaB p105/p50 (Ser337) Antibody

ABF3219 100 ug
EUR 525.6

Phospho- NF- kappaB p105/p50 (Ser893) Antibody

ABF3220 100 ug
EUR 525.6

Phospho- NF- kappaB p105/p50 (Ser907) Antibody

ABF3221 100 ug
EUR 525.6

Phospho-NF kappaB p105/p50 (Ser903) Antibody

AF8013 100ul
EUR 350
Description: Human

Phospho-NF kappaB p105/p50 (Ser923) Antibody

AF8014 100ul
EUR 350
Description: Human,Mouse,Rat

Phospho-NF kappaB p105/p50 (Ser927) Antibody

AF3217 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-NF kappaB p105/p50 (Ser932) Antibody

AF3218 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-NF kappaB p105/p50 (Ser337) Antibody

AF3219 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-NF kappaB p105/p50 (Ser893) Antibody

AF3220 100ul
EUR 280
Description: Human

Phospho-NF kappaB p105/p50 (Ser907) Antibody

AF3221 100ul
EUR 280
Description: Human

Phospho-NF kappaB p105/p50 (Ser903) Antibody

AF8013-100ul 100ul
EUR 350

Phospho-NF kappaB p105/p50 (Ser903) Antibody

AF8013-200ul 200ul
EUR 450

Phospho-NF kappaB p105/p50 (Ser923) Antibody

AF8014-100ul 100ul
EUR 350

Phospho-NF kappaB p105/p50 (Ser923) Antibody

AF8014-200ul 200ul
EUR 450

Phospho-NF kappaB p105/p50 (Ser927) Antibody

AF3217-100ul 100ul
EUR 280

Phospho-NF kappaB p105/p50 (Ser927) Antibody

AF3217-200ul 200ul
EUR 350

Phospho-NF kappaB p105/p50 (Ser932) Antibody

AF3218-100ul 100ul
EUR 280

Phospho-NF kappaB p105/p50 (Ser932) Antibody

AF3218-200ul 200ul
EUR 350

Phospho-NF kappaB p105/p50 (Ser337) Antibody

AF3219-100ul 100ul
EUR 280

Phospho-NF kappaB p105/p50 (Ser337) Antibody

AF3219-200ul 200ul
EUR 350

Phospho-NF kappaB p105/p50 (Ser893) Antibody

AF3220-100ul 100ul
EUR 280

Phospho-NF kappaB p105/p50 (Ser893) Antibody

AF3220-200ul 200ul
EUR 350

Phospho-NF kappaB p105/p50 (Ser907) Antibody

AF3221-100ul 100ul
EUR 280

Phospho-NF kappaB p105/p50 (Ser907) Antibody

AF3221-200ul 200ul
EUR 350

Phospho-NF- kappaB p105/p50 (Ser927) Antibody

E18-3217-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Phospho-NF- kappaB p105/p50 (Ser927) Antibody

E18-3217-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Phospho-NF- kappaB p105/p50 (Ser932) Antibody

E18-3218-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Phospho-NF- kappaB p105/p50 (Ser932) Antibody

E18-3218-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Phospho-NF- kappaB p105/p50 (Ser337) Antibody

E18-3219-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Phospho-NF- kappaB p105/p50 (Ser337) Antibody

E18-3219-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Phospho-NF- kappaB p105/p50 (Ser893) Antibody

E18-3220-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Phospho-NF- kappaB p105/p50 (Ser893) Antibody

E18-3220-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Phospho-NF- kappaB p105/p50 (Ser907) Antibody

E18-3221-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Phospho-NF- kappaB p105/p50 (Ser907) Antibody

E18-3221-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

NF kappaB p105 antibody

70R-51601 100 ul
EUR 344.4
Description: Purified Polyclonal NF kappaB p105 antibody

NF kappaB p105 antibody

70R-51602 100 ul
EUR 292.8
Description: Purified Polyclonal NF kappaB p105 antibody

NF kappaB p105 antibody

70R-50117 100 ul
EUR 344.4
Description: Purified Polyclonal NF kappaB p105 antibody

NF kappaB p105 antibody

70R-50118 100 ul
EUR 292.8
Description: Purified Polyclonal NF kappaB p105 antibody

AFfirm™ NF kappaB p105/p50 Mouse Monoclonal Antibody

BF8151 100ul
EUR 280
Description: Human,Mouse,Rat

AFfirm™ NF kappaB p105/p50 Mouse Monoclonal Antibody

BF8151-100ul 100ul
EUR 280

AFfirm™ NF kappaB p105/p50 Mouse Monoclonal Antibody

BF8151-200ul 200ul
EUR 350

Rabbit Polyclonal NF- kappaB p105/p50 (Ser337) Antibody (Phospho-specific)

TA325716 100 µl Ask for price

Rabbit Polyclonal NF- kappaB p105/p50 (Ser893) Antibody (Phospho-specific)

TA325717 100 µl Ask for price

Rabbit Polyclonal NF- kappaB p105/p50 (Ser907) Antibody (Phospho-specific)

TA325718 100 µl Ask for price

Rabbit Polyclonal NF- kappaB p105/p50 (Ser927) Antibody (Phospho-specific)

TA325719 100 µl Ask for price

Rabbit Polyclonal NF- kappaB p105/p50 (Ser932) Antibody (Phospho-specific)

TA325720 100 µl Ask for price

Phospho-NF kappaB p105/p50 (Ser927) Blocking Peptide

AF3217-BP 1mg
EUR 234

Phospho-NF kappaB p105/p50 (Ser932) Blocking Peptide

AF3218-BP 1mg
EUR 234

Phospho-NF kappaB p105/p50 (Ser337) Blocking Peptide

AF3219-BP 1mg
EUR 234

Phospho-NF kappaB p105/p50 (Ser893) Blocking Peptide

AF3220-BP 1mg
EUR 234

Phospho-NF kappaB p105/p50 (Ser907) Blocking Peptide

AF3221-BP 1mg
EUR 234

NF-kappaB p105/p50 Antibody

E38PA4479 100ul
EUR 225
Description: Available in various conjugation types.

NF-kappaB p105/p50 Antibody

E18-6217-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

NF-kappaB p105/p50 Antibody

E18-6217-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

NF kappaB p100 antibody

70R-50121 100 ul
EUR 292.8
Description: Purified Polyclonal NF kappaB p100 antibody

NF- kappaB p100/p52 Antibody

ABF6373 100 ug
EUR 525.6

NF kappaB p100/p52 Antibody

DF7190 200ul
EUR 420

NF kappaB p100/p52 Antibody

AF6373 100ul
EUR 280
Description: Human,Mouse,Rat

NF kappaB p100/p52 Antibody

AF6375 100ul
EUR 280
Description: Human,Mouse

NF kappaB p100/p52 Antibody

AF6373-100ul 100ul
EUR 280

NF kappaB p100/p52 Antibody

AF6373-200ul 200ul
EUR 350

NF kappaB p100/p52 Antibody

AF6375-100ul 100ul
EUR 280

NF kappaB p100/p52 Antibody

AF6375-200ul 200ul
EUR 350

Rabbit anti-NF-kappaB p105/p50 Antibody

YLD5497-100ul 100 ul
EUR 320
Description: Rabbit polyclonal antibody to NF-kappaB p105/p50

Rabbit anti-NF-kappaB p105/p50 Antibody

YLD5497-50ul 50 ul
EUR 200
Description: Rabbit polyclonal antibody to NF-kappaB p105/p50

Rabbit anti-NF-kappaB p105/p50 Antibody

YLD5498-100ul 100 ul
EUR 320
Description: Rabbit polyclonal antibody to NF-kappaB p105/p50

Rabbit anti-NF-kappaB p105/p50 Antibody

YLD5498-50ul 50 ul
EUR 200
Description: Rabbit polyclonal antibody to NF-kappaB p105/p50

Rabbit anti-NF-kappaB p105/p50 Antibody

DL93479A-100ul 100 ul
EUR 342
Description: Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Rabbit anti-NF-kappaB p105/p50 Antibody

DL93479A-50ul 50 ul
EUR 239.4
Description: Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Rabbit anti-NF-kappaB p105/p50 Antibody

DL90801A-100ul 100 ul
EUR 370
Description: Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Rabbit anti-NF-kappaB p105/p50 Antibody

DL90801A-50ul 50 ul
EUR 259
Description: Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

NF kappaB p65 antibody

70R-51627 100 ul
EUR 344.4
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-51628 100 ul
EUR 344.4
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-51629 100 ul
EUR 344.4
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-51630 100 ul
EUR 292.8
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50315 100 ul
EUR 344.4
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50316 100 ul
EUR 344.4
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50317 100 ul
EUR 344.4
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50318 100 ul
EUR 344.4
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50319 100 ul
EUR 292.8
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50320 100 ul
EUR 292.8
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50321 100 ul
EUR 292.8
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50322 100 ul
EUR 292.8
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50323 100 ul
EUR 292.8
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50324 100 ul
EUR 292.8
Description: Purified Polyclonal NF kappaB p65 antibody

NF- kappaB p65 Antibody

ABF5006 100 ug
EUR 525.6

NF- kappaB p65 Antibody

ABF6387 100 ug
EUR 525.6

NF-kappaB p105/p50 (pS337) Antibody

E38PA4122 100ul
EUR 225
Description: Available in various conjugation types.

NF-kappaB p105/p50 (pS337) Antibody

E38PA1800 100ul
EUR 225
Description: Available in various conjugation types.

Rabbit anti-NF-kappaB p105/p50 (pS337) Antibody

YLD5495-100ul 100 ul
EUR 320
Description: Rabbit polyclonal antibody to NF-kappaB p105/p50 (pS337)

Rabbit anti-NF-kappaB p105/p50 (pS337) Antibody

YLD5495-50ul 50 ul
EUR 200
Description: Rabbit polyclonal antibody to NF-kappaB p105/p50 (pS337)

Rabbit anti-NF-kappaB p105/p50 (pS337) Antibody

YLD5496-100ul 100 ul
EUR 320
Description: Rabbit polyclonal antibody to NF-kappaB p105/p50 (pS337)

Rabbit anti-NF-kappaB p105/p50 (pS337) Antibody

YLD5496-50ul 50 ul
EUR 200
Description: Rabbit polyclonal antibody to NF-kappaB p105/p50 (pS337)

Rabbit anti-NF-kappaB p105/p50 (pS337) Antibody

DL93122A-100ul 100 ul
EUR 350
Description: Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Rabbit anti-NF-kappaB p105/p50 (pS337) Antibody

DL93122A-50ul 50 ul
EUR 245
Description: Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Rabbit anti-NF-kappaB p105/p50 (pS337) Antibody

DL90800A-100ul 100 ul
EUR 350
Description: Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Rabbit anti-NF-kappaB p105/p50 (pS337) Antibody

DL90800A-50ul 50 ul
EUR 245
Description: Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Anti-Phospho- NF kappaB p65 (Ser536) Antibody

A1590-100 each
EUR 574.8

Phospho- NF- kappaB p100/p52 (Ser865) Antibody

ABF3373 100 ug
EUR 525.6

Phospho- NF- kappaB p100/p52 (Ser869) Antibody

ABF3374 100 ug
EUR 525.6

Rabbit Polyclonal NF-kappaB p105/p50 Antibody

TA325715 100 µl Ask for price

Phospho-NF kappaB p100/p52 (Ser866) Antibody

AF3373 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-NF kappaB p100/p52 (Ser870) Antibody

AF3374 100ul
EUR 280
Description: Human,Mouse,Rat

Phospho-NF kappaB p100/p52 (Ser872) Antibody

AF3375 100ul
EUR 280
Description: Human,Mouse

Phospho-NF kappaB p100/p52 (Ser866) Antibody

AF3373-100ul 100ul
EUR 280

Phospho-NF kappaB p100/p52 (Ser866) Antibody

AF3373-200ul 200ul
EUR 350

Phospho-NF kappaB p100/p52 (Ser870) Antibody

AF3374-100ul 100ul
EUR 280

Phospho-NF kappaB p100/p52 (Ser870) Antibody

AF3374-200ul 200ul
EUR 350

Phospho-NF kappaB p100/p52 (Ser872) Antibody

AF3375-100ul 100ul
EUR 280

Phospho-NF kappaB p100/p52 (Ser872) Antibody

AF3375-200ul 200ul
EUR 350

Phospho-NF- kappaB p100/p52 (Ser865) Antibody

E18-3373-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Phospho-NF- kappaB p100/p52 (Ser865) Antibody

E18-3373-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Phospho-NF- kappaB p100/p52 (Ser869) Antibody

E18-3374-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Phospho-NF- kappaB p100/p52 (Ser869) Antibody

E18-3374-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Acetyl- NF- kappaB p65 (Lys310) Antibody

ABF1017 100 ug
EUR 525.6

Phospho- NF- kappaB p65 (Ser536) Antibody

ABF2006 100 ug
EUR 525.6

Phospho- NF- kappaB p65 (Ser276) Antibody

ABF3387 100 ug
EUR 525.6

Phospho- NF- kappaB p65 (Ser529) Antibody

ABF3388 100 ug
EUR 525.6

Phospho- NF- kappaB p65 (Ser311) Antibody

ABF3389 100 ug
EUR 525.6

Phospho- NF- kappaB p65 (Ser468) Antibody

ABF3390 100 ug
EUR 525.6

Phospho- NF- kappaB p65 (Thr254) Antibody

ABF3391 100 ug
EUR 525.6

Phospho- NF- kappaB p65 (Thr435) Antibody

ABF3392 100 ug
EUR 525.6

Phospho- NF- kappaB p65 (Ser281) Antibody

ABF3393 100 ug
EUR 525.6

NF- kappaB p65 (Phospho-Ser281) Antibody

12000-100ul 100ul
EUR 302.4

NF- kappaB p65 (Phospho-Ser281) Antibody

12000-50ul 50ul
EUR 224.4

Phospho-NF- kappaB p65 (Ser536) Antibody

E18-2006-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Phospho-NF- kappaB p65 (Ser536) Antibody

E18-2006-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Phospho-NF- kappaB p65 (Ser276) Antibody

E18-3387-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Phospho-NF- kappaB p65 (Ser276) Antibody

E18-3387-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Phospho-NF- kappaB p65 (Ser529) Antibody

E18-3388-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Phospho-NF- kappaB p65 (Ser529) Antibody

E18-3388-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Phospho-NF- kappaB p65 (Ser311) Antibody

E18-3389-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Phospho-NF- kappaB p65 (Ser311) Antibody

E18-3389-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Phospho-NF- kappaB p65 (Ser468) Antibody

E18-3390-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Phospho-NF- kappaB p65 (Ser468) Antibody

E18-3390-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Phospho-NF- kappaB p65 (Thr254) Antibody

E18-3391-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Phospho-NF- kappaB p65 (Thr254) Antibody

E18-3391-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Phospho-NF- kappaB p65 (Thr435) Antibody

E18-3392-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Phospho-NF- kappaB p65 (Thr435) Antibody

E18-3392-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Thus, a common haplotype RETN tightly regulated by epigenetic mechanisms associated with p50 / p50-binding. This control is missing in the Asian haplotypes, which may have evolved to balance antagonistic effects on the protection RETN pathogen induction vs metabolism and inflammatory diseases.

Sarah

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