The Wobbler rat spinal cord motoneurons showed vacuolization especially, glial reaction, inflammation and abnormal glutamatergic parameters. Wobblers also showed a deficit of motor performance. This condition resembles amyotrophic lateral sclerosis (ALS). Wobbler mice also showed higher levels of corticosterone in the blood, adrenal and brain plus adrenal hypertrophy, suggesting that increased glucocorticoid prime chronic spinal cord nerve inflammation.
Therefore, we analyzed if treatment Wobbler mice with the glucocorticoid receptor (GR) antagonist reduced CORT113176 mentioned disorders. 30 mg / kg CORT113176 administered daily for 3 weeks reduced motoneuron vacuolation, decrease in astro and microgliosis, lowers inflammatory mediators group of high mobility box 1 protein (HMGB1), pulses like receptor 4, myeloid differentiation primary response 88 (MyD88), p50 subunit factor nuclear kappa B (NF), tumor necrosis factor (TNF) receptor, and interleukin 18 (IL18) compared with untreated Wobblers. CORT113176 improve survival signal pAkt (serine-threonine kinases) and a decrease in phosphorylated death signal Trash-N-terminal kinase (pJNK), symptoms of anti-apoptotic.
There was a moderate positive effect on glutamine synthase and astrocyte glutamate transporters, showed a decrease in glutamate excitotoxicity. In these pre-clinical studies, Wobblers receive CORT113176 show increased resistance to fatigue in the rota rod test and atrophy of the lower front leg in Week 2- 3. Therefore, the long-term treatment with attenuated CORT113176 degeneration and inflammation, improve motor performance and decrease foot deformities. Antagonism of GR may be a potential therapeutic value for neurodegenerative diseases.
Long-term effects of the glucocorticoid receptor modulator cort 113176 in murine motoneuron degeneration.
resistin expression in human monocytes is controlled by two SNPs associated promoter binding mediation NFkB p50 / p50 and C-methylation.
Resistin is a key cytokine associated with metabolic and inflammatory diseases. Especially in East Asian populations, the level of expression is strongly influenced by genetic polymorphisms. The mechanism and functional implications of this genetic control remains unknown. By using the test reporter, EMSA, the study of inhibition, sequencing of bisulfite, CHIP-Seq and gene-editing we showed that the homodimer p50 / p50 is known to act as a repressor for a number of genes of pro-inflammation plays a central role in the genetic regulation of resistin in monocytes along with promoter methylation.
RETN common haplotype in the p50 / p50 offset constitutive expression by binding to the promoter. In a variant haplotypes Asia but this interaction is disrupted by the A allele of rs3219175. SNP is a very close relationship with rs34861192, a SNP CpG, located 280 bp upstream of the supply site C-allele-specific methylation.
rs34861192 lies in the region of 100 bp was found methylated haplotype similarities but not in Asia, so that the latter have a higher basal expression, which also associates with increased histone acetylation (H3K27ac). genotype associations in cohort data from 200 East Asians revealed a significant association between this haplotype and plasma levels of factors such as TGF-b, S100B, sRAGE and IL-8 as well as the number of myeloid DC.
Description: A Rabbit Polyclonal antibody against NF?B-p105/p50 from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Description: A Rabbit Polyclonal antibody against NF?B-p105/p50 from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Description: A Rabbit Polyclonal antibody against NF?B-p105/p50 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
Description: A Rabbit Polyclonal antibody against NF?B-p105/p50 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
Description: A polyclonal antibody for detection of NF?B-p105/p50 from Human, Mouse, Rat. This NF?B-p105/p50 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p105/p50 around the non-phosphorylation site of S337
Description: A polyclonal antibody for detection of NF?B-p105/p50 from Human, Mouse, Rat. This NF?B-p105/p50 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p105/p50 around the non-phosphorylation site of S337
Description: A polyclonal antibody for detection of NF?B-p105/p50 from Human, Mouse, Rat. This NF?B-p105/p50 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p105/p50 around the non-phosphorylation site of S337
Description: A polyclonal antibody for detection of NF?B-p105/p50 from Human, Mouse. This NF?B-p105/p50 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p105/p50 around the non-phosphorylation site of S337
Description: A polyclonal antibody for detection of NF?B-p105/p50 from Human, Mouse. This NF?B-p105/p50 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p105/p50 around the non-phosphorylation site of S337
Description: A polyclonal antibody for detection of NF?B-p105/p50 from Human, Mouse. This NF?B-p105/p50 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p105/p50 around the non-phosphorylation site of S337
Description: A polyclonal antibody for detection of NF?B-p105/p50 phospho Ser337) from Human, Mouse. This NF?B-p105/p50 phospho Ser337) antibody is for WB , IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p105/p50 around the phosphorylation site of S337
Description: A polyclonal antibody for detection of NF?B-p105/p50 phospho Ser337) from Human, Mouse. This NF?B-p105/p50 phospho Ser337) antibody is for WB , IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p105/p50 around the phosphorylation site of S337
Description: A polyclonal antibody for detection of NF?B-p105/p50 phospho Ser337) from Human, Mouse. This NF?B-p105/p50 phospho Ser337) antibody is for WB , IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p105/p50 around the phosphorylation site of S337
Description: A Rabbit Polyclonal antibody against NF?B-p105/p50 (phospho Ser337) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
Description: A Rabbit Polyclonal antibody against NF?B-p105/p50 (phospho Ser337) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
Description: A Rabbit Polyclonal antibody against NF?B-p105/p50 (phospho Ser337) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Description: A Rabbit Polyclonal antibody against NF?B-p105/p50 (phospho Ser337) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Description: A polyclonal antibody for detection of NF?B-p105/p50 phospho Ser337) from Human, Mouse, Rat. This NF?B-p105/p50 phospho Ser337) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p105/p50 around the phosphorylation site of S337
Description: A polyclonal antibody for detection of NF?B-p105/p50 phospho Ser337) from Human, Mouse, Rat. This NF?B-p105/p50 phospho Ser337) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p105/p50 around the phosphorylation site of S337
Description: A polyclonal antibody for detection of NF?B-p105/p50 phospho Ser337) from Human, Mouse, Rat. This NF?B-p105/p50 phospho Ser337) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p105/p50 around the phosphorylation site of S337
Description: NFkappaB-p105/p50 is a protein encoded by the NFKB1 gene which is approximately 105,3 kDa. NFkappaB-p105/p50 is localised to the nucleus and cytoplasm. It is involved in activated TLR4 signalling, the TNFR1 pathway, toll-like receptor signalling pathways and 4-1BB pathway. It is a pleiotropic transcription factor that acts as the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. Dimers bind at kappa-B sites in the DNA of their target genes. Different dimer combinations act as transcriptional activators or repressors, respectively. NFkappaB-p105/p50 is expressed in the nervous system, liver, muscle, eye and lymph node. Mutations in the NFKB1 gene may result in common variable immunodeficiency and intestinal perforation. STJ94465 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of NFkappaB-p105/p50 protein.
Description: NFkappaB-p105/p50 is a protein encoded by the NFKB1 gene which is approximately 105,3 kDa. NFkappaB-p105/p50 is localised to the nucleus and cytoplasm. It is involved in activated TLR4 signalling, the TNFR1 pathway, toll-like receptor signalling pathways and 4-1BB pathway. It is a pleiotropic transcription factor that acts as the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. Dimers bind at kappa-B sites in the DNA of their target genes. Different dimer combinations act as transcriptional activators or repressors, respectively. NFkappaB-p105/p50 is expressed in the nervous system, liver, muscle, eye and lymph node. Mutations in the NFKB1 gene may result in common variable immunodeficiency and intestinal perforation. STJ94466 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of NFkappaB-p105/p50 protein.
Thus, a common haplotype RETN tightly regulated by epigenetic mechanisms associated with p50 / p50-binding. This control is missing in the Asian haplotypes, which may have evolved to balance antagonistic effects on the protection RETN pathogen induction vs metabolism and inflammatory diseases.
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