Salidroside Reduces Inflammation and Brain Injury After Permanent Middle Cerebral Artery Occlusion in Rats by Regulating PI3K/PKB/Nrf2/NFκB Signaling Rather than Complement C3 Activity.

Salidroside Reduces Inflammation and Brain Injury After Permanent Middle Cerebral Artery Occlusion in Rats by Regulating PI3K/PKB/Nrf2/NFκB Signaling Rather than Complement C3 Activity.

Salidroside, the active constituents of Rhodiola rosea, is nervous after a while the middle cerebral artery occlusion (tMCAO). However, the impact in other experimental models of stroke are poorly understood. Here, we examined the effects of daily intraperitoneal injections salidroside in rats after permanent MCAO (pMCAO). the volume of cerebral infarction in 1 day after pMCAO was significantly reduced by treatment with 100 mg / kg / day salidroside, but not at 25 or 50 mg / kg / day, and benefits from salidroside increased significantly for at least 7 days of treatment, when it also accompanied by neurologic deficit score decreased.

These observations prompted us to investigate the underlying mechanisms of action salidroside. 100 mg / kg for 1 day salidroside increased Neun, Nrf2 and HO-1 downstream mediator, while reducing NF nuclear p50, IL-6, and TNF. Brusatol, Nrf2 inhibitor, blocked the action salidroside on Nrf2, NF p50, IL-6, and TNF.

Salidroside also increase the ratio of p-PKB / PKB at 1 day after pMCAO even before brusatol. LY294002, PI3K inhibitors, to prevent these effects from salidroside, including those in Neun, p-PKB / PKB, Nrf2, HO-1, and pro-inflammatory mediators. Instead, salidroside no significant effect on brain levels of complement C3 after pMCAO, or on C3 activity as measured by the expression of cerebral Egr1.

Therefore, our findings suggest that salidroside reduce neuroinflammation and neuronal damage by regulating the PI3K / PKB / Nrf2 / NF signaling pathways after pMCAO, and that this neuroprotective effect does not involve modulation of complement C3 activity.

Salidroside Reduces Inflammation and Brain Injury After Permanent Middle Cerebral Artery Occlusion in Rats by Regulating PI3K/PKB/Nrf2/NFκB Signaling Rather than Complement C3 Activity.
Salidroside Reduces Inflammation and Brain Injury After Permanent Middle Cerebral Artery Occlusion in Rats by Regulating PI3K/PKB/Nrf2/NFκB Signaling Rather than Complement C3 Activity.

Identification of the canonical NF (C-NF) pathway in uveal melanoma and its relation to patient outcomes.

Inflammation of the uveal melanoma (UM) associated with a poor prognosis. This is a rare type of cancer, in which the metastasis is usually fatal within a year. Infiltration with inflammatory infiltration rise to the development of the disease but does not seem to inhibit metastasis. The Canonical NF (C-NF) pathway is known to play an important role in tumor inflammation.

Therefore we, studying the canonical NF protein expression and their prognostic relevance in UM. Our study evaluated the expression of the protein C-NF (p65, p50 and c-Rel) using immunohistochemistry on sections of formalin-fixed 75 MW. Activation of NF subunit is determined on fresh tumor specimens by measuring the activity of DNA-binding core using ELISA NF test. Real-time PCR was performed on frozen matter on 58 tumor. The presence of heterodimers original C-NF (p65 / p50 and c-Rel / p50) was confirmed by co-immunoprecipitation followed by Western blotting.

We observed a high nuclear immunoreactivity of p65, p50 and c-Rel protein in 54, 60 and 41% of cases MW, respectively. C-NF protein expression significantly correlated with environmental parameters associated with inflammation of the UM. Nuclear immunoreactivity of p65 and p50 was associated with lower patient survival (p = 0.041; p = 0.048), whereas c-Rel are not. Our findings suggest that protein C-NF expressed more often in UM with inflammation than those without inflammation. Activation of NF canonical pathway more often in high-risk patients with UM.

Anti- cRel (NFkB) Antibody

GWB-393707 0.1 ml Ask for price

Anti-NFKB p50 (NFKB1) Antibody

GWB-CC6745 100 ul Ask for price

Rabbit Anti NFkB p65 (pSer276)

MBS225571-005mL 0.05mL
EUR 455

Rabbit Anti NFkB p65 (pSer276)

MBS225571-5x005mL 5×0.05mL
EUR 1870

Rabbit Anti NFkB p65 (pSer468)

MBS225573-005mL 0.05mL
EUR 455

Rabbit Anti NFkB p65 (pSer468)

MBS225573-5x005mL 5×0.05mL
EUR 1870

Rabbit anti NFkB p105 (pSer907)

MBS225294-005mL 0.05mL
EUR 455

Rabbit anti NFkB p105 (pSer907)

MBS225294-5x005mL 5×0.05mL
EUR 1870

Rabbit anti NFkB p105 (pSer927)

MBS225295-005mL 0.05mL
EUR 455

Rabbit anti NFkB p105 (pSer927)

MBS225295-5x005mL 5×0.05mL
EUR 1870

Rabbit anti NFkB p105 (pSer932)

MBS225296-005mL 0.05mL
EUR 455

Rabbit anti NFkB p105 (pSer932)

MBS225296-5x005mL 5×0.05mL
EUR 1870

Rabbit anti NFkB p100 (pSer870)

MBS225297-005mL 0.05mL
EUR 455

Rabbit anti NFkB p100 (pSer870)

MBS225297-5x005mL 5×0.05mL
EUR 1870

Rabbit anti NFkB-p65 (pSer311)

pAP11260 100ul
EUR 326.4

Anti-NFkB/NFKB2 p100/p52 Antibody

PA1946 100ug/vial
EUR 154
Description: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat;Western blot, 0.1-0.5μg/ml, Mouse, Rat

Anti-NFkB p100/p52/NFKB2 Antibody

PA1946-1 100ug/vial
EUR 352.8

Anti-NFkB/NFKB1 p105/p50 Antibody

PA2111 100ug/vial
EUR 154
Description: Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Anti-NFkB/NFKB1 p105/p50 Antibody

PA2111-1 100ug/vial
EUR 154
Description: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Anti-NFkB p100/NFKB2 Rabbit pAb

GB110053 100 μL
EUR 100

Anti- NFkB p65 (Rel A) Antibody

GWB-34C0DE 0.1 ml Ask for price

Rabbit anti NFkB-p105/p50 (pSer932)

pAP11251 100ul
EUR 326.4

NFkB Antibody

GWB-A99D1B 0.1 ml Ask for price

Anti-NFkB-p100 (Ab-866) NFKB2 Antibody

A01228-1 100ul
EUR 360
Description: Boster Bio Anti-NFkB-p100 (Ab-866) NFKB2 Antibody (Catalog # A01228-1). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Anti-NFkB-p105 (Ab-927) NFKB1 Antibody

A00283-1 100ul
EUR 360
Description: Boster Bio Anti-NFkB-p105 (Ab-927) NFKB1 Antibody (Catalog # A00283-1). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Rabbit anti NFkB p65 Polyclonal Antibody

MBS460145-01mg 0.1mg
EUR 320

Rabbit anti NFkB p65 Polyclonal Antibody

MBS460145-5x01mg 5×0.1mg
EUR 1390

Rabbit anti NFkB p50 Polyclonal Antibody

MBS460184-01mg 0.1mg
EUR 320

Rabbit anti NFkB p50 Polyclonal Antibody

MBS460184-5x01mg 5×0.1mg
EUR 1390

Rabbit anti NFkB p65 Polyclonal Antibody

TA354228 100 µg Ask for price

Rabbit anti NFkB p50 Polyclonal Antibody

TA354277 100 µg Ask for price

RABBIT ANTI HUMAN NFkB p65 (pSer536)

MBS222826-005mg 0.05mg
EUR 525

RABBIT ANTI HUMAN NFkB p65 (pSer536)

MBS222826-5x005mg 5×0.05mg
EUR 2185

Rabbit Anti-Human NFkB p65 Polyclonal

SPC-1279D 0.1ml
EUR 286
Description: A polyclonal antibody for NFkB p65 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human NF-kappaB p65. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:2000), IHC (1:200), ICC/IF (1:200). This NFkB p65 antibody is unconjugated.

Rabbit Anti-Human NFkB p65 Polyclonal

SPC-1279D-A390 0.1ml
EUR 340
Description: A polyclonal antibody for NFkB p65 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human NF-kappaB p65. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:2000), IHC (1:200), ICC/IF (1:200). This NFkB p65 antibody is conjugated to ATTO 390.

Rabbit Anti-Human NFkB p65 Polyclonal

SPC-1279D-A488 0.1ml
EUR 339
Description: A polyclonal antibody for NFkB p65 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human NF-kappaB p65. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:2000), IHC (1:200), ICC/IF (1:200). This NFkB p65 antibody is conjugated to ATTO 488.

Rabbit Anti-Human NFkB p65 Polyclonal

SPC-1279D-A594 0.1ml
EUR 339
Description: A polyclonal antibody for NFkB p65 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human NF-kappaB p65. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:2000), IHC (1:200), ICC/IF (1:200). This NFkB p65 antibody is conjugated to ATTO 594.

Rabbit Anti-Human NFkB p65 Polyclonal

SPC-1279D-BI 0.1ml
EUR 334
Description: A polyclonal antibody for NFkB p65 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human NF-kappaB p65. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:2000), IHC (1:200), ICC/IF (1:200). This NFkB p65 antibody is conjugated to Biotin.

Rabbit Anti-Human NFkB p65 Polyclonal

SPC-1279D-FITC 0.1ml
EUR 330
Description: A polyclonal antibody for NFkB p65 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human NF-kappaB p65. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:2000), IHC (1:200), ICC/IF (1:200). This NFkB p65 antibody is conjugated to FITC.

Anti-RELA/NFKB p65 Antibody (clone 8G3)

MBS2401625-005mg 0.05mg
EUR 595

Anti-RELA/NFKB p65 Antibody (clone 8G3)

MBS2401625-5x005mg 5×0.05mg
EUR 2500

Anti-NFkB/NFKB1 p105/p50 Antibody Picoband™

PB9149 100ug/vial
EUR 154
Description: Western blot, 0.1-0.5μg/ml, Human;_x000D_Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human;_x000D_Flow Cytometry, 1-3 μg/1×106 cells, Human;_x000D_

Anti-NFkB/NFKB2 p100/p52 Antibody Picoband™

PB9150 100ug/vial
EUR 154
Description: Western blot, 0.1-0.5μg/ml, Human, Mouse;_x000D_Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat

Anti- NFkB p65 (N-terminal specific) Antibody

GWB-EDFAF1 0.1 ml Ask for price

NFkB p50 Antibody

3354R-100 each
EUR 379.2

NFkB p50 Antibody

3354R-30T each
EUR 175.2

NFkB p65 antibody

70R-36057 100 ug
EUR 294
Description: Rabbit polyclonal NFkB p65 antibody

NFkB p65 antibody

70R-37450 100 ug
EUR 260
Description: Rabbit Polyclonal NFkB p65 antibody

NFkB p65 antibody

70R-37451 100 ug
EUR 260
Description: Rabbit Polyclonal NFkB p65 antibody

NFkB p65 antibody

70R-37452 100 ug
EUR 260
Description: Rabbit Polyclonal NFkB p65 antibody

NFkB p65 antibody

70R-37453 100 ug
EUR 260
Description: Rabbit Polyclonal NFkB p65 antibody

NFkB p65 antibody

70R-37454 100 ug
EUR 260
Description: Rabbit Polyclonal NFkB p65 antibody

NFkB p65 antibody

70R-11575 100 ug
EUR 343
Description: Rabbit polyclonal NFkB p65 antibody

NFkB p50 antibody

70R-11768 100 ug
EUR 343
Description: Rabbit polyclonal NFkB p50 antibody

NFkB p65 Antibody

3038-100 each
EUR 379.2

NFkB p65 Antibody

3038-30T each
EUR 175.2

NFkB p65 antibody

20R-1437 100 ug
EUR 807.6
Description: Rabbit polyclonal NFkB p65 antibody

NFkB p50 antibody

20R-1520 100 ug
EUR 807.6
Description: Rabbit polyclonal NFkB p50 antibody

NFKB p65 antibody

20R-2196 50 ug
EUR 269
Description: Rabbit polyclonal NFKB p65 antibody

NFKB p65 antibody

20R-2913 100 ul
EUR 471.6
Description: Rabbit polyclonal NFkB p65 antibody

NFKB p65 antibody

10R-1917 100 ul
EUR 435
Description: Mouse monoclonal NFKB p65 antibody

NFkB p65 antibody

10R-6718 100 ug
EUR 859.2
Description: Mouse monoclonal NFkB p65 antibody

NFkB p65 antibody

10R-7999 100 ug
EUR 630
Description: Mouse monoclonal NFkB p65 antibody

NFKB P52 antibody

20-PR79 100 µL
EUR 748
Description: Rabbit polyclonal NFKB P52 antibody

NFkB p52 antibody

10R-8543 100 ul
EUR 471.6
Description: Mouse monoclonal NFkB p52 antibody

NFkB p65 Antibody

abx012874-100g 100 µg
EUR 237.5

NFkB p65 Antibody

20-abx012874
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  • 10 ug
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  • 300 µg

NFkB p65 Antibody

abx012874-10g 10 µg
EUR 43.75

NFkB p65 Antibody

abx012874-200g 200 µg
EUR 312.5

NFkB p65 Antibody

20-abx008774
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  • 30 ul
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NFkB p65 Antibody

20-abx008775
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  • 30 ul
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NFkB p65 Antibody

20-abx008776
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  • 30 ul
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NFkB p65 Antibody

20-abx008777
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  • 30 ul
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NFkB p65 Antibody

20-abx008778
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  • 30 ul
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NFkB p65 Antibody

20-abx008779
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  • 30 ul
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NFkB p65 Antibody

abx012273-100g 100 µg Ask for price

NFkB p65 Antibody

abx012273-100ul 100 ul
EUR 493.2

NFkB p65 Antibody

abx012273-10g 10 µg
EUR 362.5

NFkB p65 Antibody

abx012273-200g 200 µg Ask for price

NFkB p65 Antibody

20-abx133308
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NFkB p65 Antibody

20-abx134504
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  • 30 ul
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NFkB p65 Antibody

20-abx134505
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  • 30 ul
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NFkB p65 Antibody

20-abx134506
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  • 30 ul
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NFkB p65 Antibody

GWB-954311 0.1 ml Ask for price

NFkB p65, Antibody

GWB-B0BC81 1 ml Ask for price

NFkB p50, Antibody

GWB-F8446B 7 ml Ask for price

NFkB p65 Antibody

GWB-FDFDFB 0.05 ml Ask for price

NFkB p65, Antibody

GWB-C15EEA 7 ml Ask for price

NFkB p50 Antibody

GWB-516B45 0.1 ml Ask for price

NFkB p50, Antibody

GWB-549338 1 ml Ask for price

NFkB p50 Antibody

GWB-57C6C0 0.05 ml Ask for price

NFkB p65 Antibody

GWB-59AB2A 0.1 mg Ask for price

NFkB p65 Antibody

E2220013 100ug
EUR 225
Description: Available in various conjugation types.

NFkB P65 Antibody

E76020 100μl
EUR 225
Description: Available in various conjugation types.

NFkB p65 Antibody

MBS5313790-01mg 0.1mg
EUR 420

NFkB p65 Antibody

MBS5313790-5x01mg 5×0.1mg
EUR 1740

NFkB p65 Antibody

MBS5313791-01mg 0.1mg
EUR 420

NFkB p65 Antibody

MBS5313791-5x01mg 5×0.1mg
EUR 1740

NFkB p65 Antibody

MBS5313792-01mg 0.1mg
EUR 420

NFkB p65 Antibody

MBS5313792-5x01mg 5×0.1mg
EUR 1740

NFkB p65 Antibody

MBS5313793-01mg 0.1mg
EUR 420

NFkB p65 Antibody

MBS5313793-5x01mg 5×0.1mg
EUR 1740

NFkB p65 Antibody

MBS5313794-01mg 0.1mg
EUR 420

NFkB p65 Antibody

MBS5313794-5x01mg 5×0.1mg
EUR 1740

NFKB p65 antibody

MBS5309652-005mg 0.05mg
EUR 485

NFKB p65 antibody

MBS5309652-5x005mg 5×0.05mg
EUR 2045

NFkB p65 Antibody

MBS5311137-01mg 0.1mg
EUR 470

NFkB p65 Antibody

MBS5311137-5x01mg 5×0.1mg
EUR 2080

NFkB p50 Antibody

MBS5311271-01mg 0.1mg
EUR 470

NFkB p50 Antibody

MBS5311271-5x01mg 5×0.1mg
EUR 2080

NFKB P52 antibody

MBS534424-01mL 0.1mL
EUR 1355

NFKB P52 antibody

MBS534424-5x01mL 5×0.1mL
EUR 5945

NFkB P65 Antibody

MBS857741-01mL 0.1mL
EUR 305

NFkB P65 Antibody

MBS857741-01mLAF405L 0.1mL(AF405L)
EUR 565

NFkB P65 Antibody

MBS857741-01mLAF405S 0.1mL(AF405S)
EUR 565

NFkB P65 Antibody

MBS857741-01mLAF610 0.1mL(AF610)
EUR 565

NFkB P65 Antibody

MBS857741-01mLAF635 0.1mL(AF635)
EUR 565

NFKB p65 antibody

MBS830878-01mL 0.1mL
EUR 745

NFKB p65 antibody

MBS830878-5x01mL 5×0.1mL
EUR 3205

NFkB p65 Antibody

MBS8504743-01mg 0.1mg
EUR 305

NFkB p65 Antibody

MBS8504743-01mLAF405L 0.1mL(AF405L)
EUR 565

NFkB p65 Antibody

MBS8504743-01mLAF405S 0.1mL(AF405S)
EUR 565

NFkB p65 Antibody

MBS8504743-01mLAF610 0.1mL(AF610)
EUR 565

NFkB p65 Antibody

MBS8504743-01mLAF635 0.1mL(AF635)
EUR 565

NFkB p105 Antibody

3897-200 each
EUR 379.2

NFkB p105 Antibody

3897-30T each
EUR 175.2

NFkB p100 Antibody

49633 100ul
EUR 499

NFkB p100 Antibody

49633-100ul 100ul
EUR 399.6

NFkB p100 Antibody

49633-50ul 50ul
EUR 286.8

NFkB p100 antibody

70R-35337 100 ug
EUR 294
Description: Purified Rabbit polyclonal NFkB p100 antibody

NFKB p105 antibody

70R-35682 100 ug
EUR 294
Description: Rabbit polyclonal NFKB p105 antibody

NFkB p105 antibody

70R-12174 200 ug
EUR 343
Description: Rabbit polyclonal NFkB p105 antibody

NFkB p105 antibody

20R-1755 200 ug
EUR 807.6
Description: Rabbit polyclonal NFkB p105 antibody

NFKB p105 antibody

20R-2086 50 ug
EUR 269
Description: Rabbit polyclonal NFKB p105 antibody

NFKB p105 antibody

20R-2132 50 ug
EUR 269
Description: Rabbit polyclonal NFKB p105 antibody

NFKB p100 antibody

20R-2199 50 ug
EUR 269
Description: Rabbit polyclonal NFKB p100 antibody

NFKB p100 antibody

20R-2200 50 ug
EUR 269
Description: Rabbit polyclonal NFKB p100 antibody

NFKB p105 antibody

20R-2201 50 ug
EUR 269
Description: Rabbit polyclonal NFKB p105 antibody

NFKB p105 antibody

20R-2202 50 ug
EUR 269
Description: Rabbit polyclonal NFKB p105 antibody

NFKB p105 antibody

20R-2203 50 ug
EUR 269
Description: Rabbit polyclonal NFKB p105 antibody

NFKB p105 antibody

20R-2402 50 ug
EUR 269
Description: Rabbit polyclonal NFKB p105 antibody

NFKB p100 antibody

20R-2450 50 ug
EUR 269
Description: Rabbit polyclonal NFKB p100 antibody

NFKB p105 antibody

20R-2459 50 ug
EUR 269
Description: Rabbit polyclonal NFKB p105 antibody

NFkB p105 Antibody

20-abx007474
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NFkB p100 Antibody

20-abx008937
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  • 30 ul
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NFkB p105 Antibody

20-abx008939
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  • 30 ul
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NFkB p105 Antibody

20-abx133154
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  • 30 ul
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NFkB p100 Antibody

20-abx133260
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  • 30 ul
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NFkB p100 Antibody

MBS9436376-005mL 0.05mL
EUR 325

This observation may help to understand the behavior of high-risk tumors, the upregulation of the protein C-NF NF contributes to tumor aggressiveness.The RELA members of family of transcription factors, p50, and cRel form of homo and heterodimers are inhibited by IκBα, IκBβ, and IκBε. NF members of this family have diverse biological functions, and they are different expression profiles, leading to different concentrations in different tissue types.

Sarah

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